MALATE OXIDATION BY ARUM SPADIX MITOCHONDRIA: PARTICIPATION AND CHARACTERISTICS OF NAD MALIC ENZYME *
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منابع مشابه
Malate Dehydrogenase and NAD Malic Enzyme in the Oxidation of Malate by Sweet Potato Mitochondria.
Over a range of concentrations from less than 0.1 mm to more than 70 mm, sweet potato root mitochondria display a bimodal substrate saturation isotherm for malate. The high affinity portion of the isotherm has an apparent Km for malate of 0.85 mm and fits a rectangular hyperbolic function. The low affinity portion of the isotherm is sigmoid in character and gives an apparent S(0.5) of 40.6 mm a...
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MALATE OXIDATION IN PLANT MITOCHONDRIA PROCEEDS THROUGH THE ACTIVITIES OF TWO ENZYMES: a malate dehydrogenase and a NAD(+)-dependent malic enzyme. In cauliflower, mitochondria malate oxidation via malate dehydrogenase is rotenone- and cyanide-sensitive. Addition of exogenous NAD(+) stimulates the oxidation of malate via malic enzyme and generates an electron flux that is both rotenone- and cyan...
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5. A Fe2+ ion-ethylenediaminetetra-acetic acid complex can catalyse the oxidative formation of an inhibitor from schradan. 6. A mechanism for the simultaneous oxidation of schradan and reduced triphosphopyridine nucleotide is suggested. 7. The requirements for the conversion of dimefox to an anti-esterase are the same as those for schradan, but the rate of esterase inhibition is much higher. Di...
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Mitochondria isolated from pea leaves (Pisum sativum L.) readily oxidized malate and glycine as substrates. The addition of glycine to mitochondria oxidizing malate in state 3 diminished the rate of malate oxidation. When glycine was added to mitochondria oxidizing malate in state 4, however, the rate of malate oxidation was either unaffected or stimulated. The reason both glycine and malate ca...
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NAD-dependent malic enzyme (EC 1.1.1.39) was obtained from isolated mitochondria of cauliflower buds (Brassica oleracea L., var. botrytis). The NAD-linked activity is accompanied by a minor NADP-linked activity. Some contaminant NADP-malic enzyme from the supernatant and the plasma membrane is usually present in crude mitochondrial preparations. NAD-dependent malic enzyme has been purified 38-f...
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ژورنال
عنوان ژورنال: New Phytologist
سال: 1984
ISSN: 0028-646X,1469-8137
DOI: 10.1111/j.1469-8137.1984.tb03584.x